Fructosyl peptide oxidase

Details Fructosyl peptide oxidase Fructosyl peptide oxidase

2006-03-28

2006-03-28

Rao, Manjunath (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S004000, C435S006120, C435S440000, C435S252300, C435S320100, C435S069100, C435S071100, C435S025000, C536S023200, C536S023700

Reexamination Certificate

active

07018823

ABSTRACT:
The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase.A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.

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Sequence Alignment—Yoshida et al. oxidase.
N. Yoshida, et al., European Journal of Biochemistry, vol. 242, No. 3, pp. 499-505, XP-002089646, “Primary Structures of Fungal Fructosyl Amino Acid Oxidases and Their Application to the Measurement of Glycated Proteins”, Dec. 15, 1996.
N. Yoshida, et al., Applied and Environmental Microbiology, vol. 61, No. 12, pp. 4487-4489, XP-000561863, “Distribution and Properties of Fructosyl Amino Acid Oxidase in Fungi”, Dec. 1, 1995.

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