Fructose-1,6-bisphosphate aldolase, a process for the preparatio

Details Fructose-1,6-bisphosphate aldolase, a process for the preparatio Fructose-1,6-bisphosphate aldolase, a process for the preparatio

1990-12-07

1992-11-10

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435 72, 435183, 435882, C12N 988, C12N 120, C12P 1900, C12P 1926

Patent

active

051622211

ABSTRACT:
A fructose-1,6-bisphosphate aldolase with obtained from Staphylococcus carnosus is disclosed. The aldolase has considerably improved stability as compared to aldolase from rabbit muscle, having an inactivation rate of 0.77%/d at 25.degree. C. as compared with 59.3%/d for rabbit muscle aldolase. The aldolase is obtained by culturing Staphylococcus carnosus cells, mechanically disrupting the cell mass, and then working up the product by fractional ammonium sulfate precipitation, pH fractionation and ion exchange chromatography to provide F-1,6-BP aldolase with a specific activity of 25 U/mg in 21% yield. Particularly high yields are obtained by the aqueous 2-phase extraction and obtaining the enzyme from the upper phase by anion exchange chromatography. The aldolase is suitable for synthesis of carbohydrates and derivatives thereof by enzymatic reaction of aldehydes with DHAP.

REFERENCES:
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patent: 4958212 (1990-09-01), Stancesco et al.
patent: 4966856 (1990-10-01), Ito et al.
Serianni et al., "Enzymic Synthesis of C-Enriched Aldoses, Ketoses, and Their Phosphate Esters", Methods in Enzymology, vol. 89, pp. 83-92.
Gotz et al., "Purification and Characterisation of an Unusually Heat-Stable and Acid/Base-Stable Class I Fructose-1,6-bisphosphate Aldolase from Staphylococcus aureus", Eur. J. Biochem., 108, 1980, pp. 295-301.
Schleifer et al., "Description of a New Species of the Genus Staphylococcus; Staphylococcus carnosus", International Journal of Systematic Bacteriology, Apr. 1982, vol. 32, No. 2, pp. 153-156.

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