Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters carbohydrate production in the plant
Patent
1997-04-05
2000-02-29
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters carbohydrate production in the plant
800263, 800286, 800287, 800298, 8003174, 435101, 435194, 435468, 536 236, 536 241, 536 245, C12N 1529, C12N 1554, C12N 1582, C12P 1904, A01H 500
Patent
active
060311541
ABSTRACT:
This invention provides for novel cDNA and regulatory DNA sequences which modify carbohydrate metabolism in ripening fruit. In order to suppress fructokinase gene expression in transgenic plants and their fruit, we have isolated the cDNA of two tomato fructokinase genes that are expressed in developing and ripening fruit. These cDNA sequences, antisense sequences or ribozymes can be used to suppress expression of the transgenic plant's endogenous genes in ripening fruit by the use of chimeric constructions of the cDNA or antisense using either constitutive or fruit-specific and ripening-regulating regulatory sequences.
In addition to a novel method of modifying carbohydrate metabolism, we have also found a novel fructokinase gene. Of the two tomato fructokinase genes described in this invention, one is highly homologous to previously described potato fructokinase gene; the other is highly divergent and represents a kinetically unique plant fructokinase.
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Bennett Alan B.
Kanayama Yoshinori
Fox David T.
The Regents of the University of California
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