Frozen spermatozoa compositions and uses thereof

Details Frozen spermatozoa compositions and uses thereof Frozen spermatozoa compositions and uses thereof

2006-11-21

2006-11-21

Witz, Jean C. (Department: 1651)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

C435S001300

Reexamination Certificate

active

07138227

ABSTRACT:
The present invention provides a composition for freezing or freeze-drying spermatozoa or sperm heads thereof, wherein the composition enables the spermatozoa or sperm heads thereof to maintain chromosome integrity at a temperature of about +4° C. to about −200° C. Also provided is a container containing the composition. The present invention further provides a method for maintaining chromosome integrity of spermatozoa or sperm heads during freezing or freeze-drying. The present invention also provides methods for freezing or freeze-drying spermatozoa to obtain at least one spermatozoan capable of fertilizing an oocyte to produce a live offspring. Also provided are frozen or freeze-dried spermatozoa produced by these methods, and containers containing the frozen or freeze-dried spermatozoa. Finally, the present invention provides methods for producing a live mammalian offspring from an oocyte fertilized with a rehydrated freeze-dried (or thawed or freeze-thawed) spermatozoan.

REFERENCES:
patent: 5773217 (1998-06-01), Wangh
Aisen et al., Theriogenology 53: 1053-1061 (2000).
Aisen et al., “Effect of Trehalose and EDTA on Cryoprotective Action of Ram Semen Diluents” Theriogenology, 53(5): 1053-61, 2000.
Carroll et al., “Normal Fertilization and Development of Frozen-Thawed Mouse Oocytes: Protective Action of Certain Macromoleculaes” Biol. Reprod., 48:606-12, 1993.
Chatot et al., “Development of 1-Cell Embryos from Different Strains of Mice in CZB Medium” Biol. Reprod., 42:432-40, 1990.
Chatot et al., “An Improved Culture Medium Supports Development of Random-bred 1-Cell Mouse Embryos in vitro” J. Reprod. Fertil., 86:679-88, 1989.
Downs and Mastropolo, “The Participation of Energy Substrates in the Control of Meiotic Maturation in Murine Oocytes” Develop. Biol., 162:154-68, 1994.
Davis et al., “Basic Methods in Molecular Biology”, New York: Elsevier, 1986, p. 47 (N/A).
Fairbairn et al., “The Comet Assay: A Comprehensive Review” Mutat. Res., 339:37-59, 1995.
Groigno and Whitaker, “An Anaphase Calcium Signal Controls Chromosome Disjunction in Early Sea Urchin Embryos” Cell, 92:193-204, 1998.
Izant, J.G., “The Role of Calcium Ions During Mitosis” Chromosoma, 88:1-10, 1983.
Kamiguchi and Mikamo, “An Improved, Efficient Method for Analyzing Human Sperm Chromosomes Using Zona-Free Hamster Ova” Am. J. Hum. Genet., 38:724-40, 1986.
Katoh et al., Jpn. J. Genet., 56:357-63, 1981 (N/A).
Kimura and Yanagimachi, “Intracytoplasmic Sperm Injection in the Mouse” Biol. Reprod., 52:709-20, 1995.
Kishikawa et al., “Chromosome Analysis of BALB/c Mouse Spermatozoa with Normal and Abnormal Head Morphology” Biol. Reprod., 61:809-12, 1999.
Kundu et al., “Effect of Dextrans on Cryopreservation of Goat Cauda Epididymal Spermatozoa Using a Chemically Defined Medium” Reproduction, 123 (6):907-13, 2002.
Kuretake et al., “Fertilization and Development of Mouse Oocytes Injectes with Isolated Sperm Heads” Biol. of Reprod., 55:789-95, 1996.
Kusakabe et al., “Detection of Neocarzinostatin-induced Translocations in Human Sperm Chromosomes Using Fluorescence in situ Hybridization of Chromosome 2” Mutat. Res., 369:51-58, 1996.
Maione et al., “Activation of Endogenous Nucleases in Mature Sperm Cells Upon Interaction with Exogenous DNA” DNA Cell Biol., 16:1087-97, 1997.
Martin, et al., “Human Sperm Chromosome Complements after Microinjection of Hamster Eggs” J. Reprod. Fert., 84:179-86, 1988.
Mazur et al., The Enhancement of the Ability of Mouse Sperm to Survive Freezing and Thawing by the Use of High Concentrations of Glycerol and the Presence of anEscherichia coliMembrane Preparation (Oxyrase) to Lower the Oxygen Concentration Cryobiology, 40:187-209, 2000.
Nakagata et al., Exp. Anim., 42:317-20, 1993 (N/A).
Nakagata, “Cryopreservation of Mouse Spermatozoa” Mammalian Genome, 11:572-76, 2000.
Nakagata et al., “Positive Effect of Partial Zona-Pellucida Dissection on the In Vitro Fertilizing Capacity of Cryopreserved C57BL/6J Transgenic Mouse Spermatozoa of Low Mobility” Biol. Reprod., 57:1050-55, 1997.
Nolan et al., “Implementation of a Large-Scale ENU Mutagenesis Program: Towards Increasing the Mouse Mutant Resource” Mammalian Genome, 11:500-06, 2000.
Ohsako et al., “Comparison of the Nuclear DNA Stability Against Freezing-Thawing and High Temperature Treatments Between Spermatozoa and Somatic Cells” J. Vet. Med. Sci., 59:1085-88, 1997.
Parrington et al., Curr. Topics Dev. Biol., 39:215-43, 1998 (N/A).
Rodriguez et al., “Nuclear Chromatin Decondensation of Spermatozoa in Vitro: A Method for Evaluating the Fertilizing Ability of Ovine Semen” Int. J. Androl., 8(2):147-58, 1985.
Ross et al., “Repeated Freezing and Thawing of Peripheral Blood and DNA in Suspension: Effects on DNA Yield and Integrity” J. Med. Genet., 27:569-70, 1990.
Russo et al., “Further Evidence for the Aneuploidogenic Properties of Chelating Agents: Induction of Micronuclei in Mouse Male Germ Cells by EDTA” Environ. Mol. Mutagen., 19(2):125-31, 1992.
Rybouchkin et al., “Unprotected Freezing of Human Spermatozoa Exerts a Detrimental Effect on Their Oocyte Activating Capacity and Chromosome Integrity” Zygote, 4:263-68, 1996.
Singh et al., “A Simple Technique for Quantitation of Low Levels of DNA Damage in Individuals Cells” Exp. Cell Res., 175:184-91, 1988.
Steele et al., “Comparison of the Effects of Two Methods of Cryopreservation of Testicular Sperm DNA” Fertil. Steril., 74:450-53, 2000.
Sztein et al., “In vitro Fertilization with Cryopreserved Inbred Mouse Sperm” Biol. Reprod., 63:1774-80, 2000.
Sztein et al., “Motility of Cryopreserved Mouse Spermatozoa Affected by Temperature of Collection and Rate of Thawing” Cryobiology, 35:46-52, 1997.
Tada et al., “Cryopreservation of Mouse Spermatozoa in the Presence of Raffinose and Glycerol” J. Reprod. Fertil., 89:511-16, 1990.
Tanaka et al., “Formation of Chromosome-type Alberrations at the First Cleavage After MMS Treatment in Late Spermatids of Mice” Cytogenet. Cell Genet., 31:145-52, 1981.
Tateno et al., “Sonication Per Se Is Not as Deleterious to Sperm Chromosomes as Previously Inferred” Biol. Reprod., 63:341-46, 2000.
Thornton et al., “Large Numbers of Mice Established by in Vitro Fertilization with Cryopreserved Spermatozoa: Implications and Applications for Genetic Resource Banks, Mutagenesis Screens, and Mouse Backcrosses”.
Visvardis et al., “Study of DNA Damage Induction and Repair Capacity of Fresh and Cryopreserved Lymphocytes Exposed to H2O2 and gamma-Irradiation With the Alkaline Comet Assay” Mutat. Res., 383:71-80, 1997.
Vishwanath et al., “Do Sperm Cells Age? A Review of the Physiological Changes in Sperm During Storage at Ambient Temperature” Reprod. Fertil. Dev., 9(3):321-31, 1999.
Wakayama et al., “Production of Normal Offspring From Mouse Oocytes Injected With Spermatozoa Cryopreserved With or Without Cryoprotection” J. Reprod. Fert., 112(1):11-17, 1996.
Wakayama and Yanagimachi, “Development of Normal Mice From Oocytes Injected With Freeze-Dried Spermatozoa” Nat. Biotechnol., 16:639-41, 1998.
Wakayama et al., “Production of Normal Offspring From Mouse Oocytes Injected With Spermatozoa Cryopreserved With or Without Cryoprotection” J. Reprod. Fert., 112:11-17, 1998.
Whittingham et al., “Long-term Storage of Mouse Embryos at—196C: the Effect of Background Radiation” Genet. Res. (Camb.), 29:171-81, 1977.
Wood et al., “High Rates of Survival and Fertilization of Mouse and Hamster Oocytes After Vitrification in Dimethylsulphoxide” Biol. Reprod., 49:489-95, 1993.
Yu et al., “4-Bromo-7-Hydroxyindan Oxime—a N

Affiliated with

Also associated with

Rating

Frozen spermatozoa compositions and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Frozen spermatozoa compositions and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Frozen spermatozoa compositions and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3687511