Radiant energy – Ionic separation or analysis – With sample supply means
Reexamination Certificate
2008-01-08
2008-01-08
Berman, Jack (Department: 2881)
Radiant energy
Ionic separation or analysis
With sample supply means
C250S281000, C250S282000, C250S287000, C436S161000, C436S007000, C436S007000, C210S198200, C210S635000
Reexamination Certificate
active
07317187
ABSTRACT:
Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.
REFERENCES:
patent: 6175112 (2001-01-01), Karger et al.
patent: 6627453 (2003-09-01), Hindsgaul et al.
patent: 6723235 (2004-04-01), Hindsgaul et al.
patent: 2002/0150535 (2002-10-01), Madras et al.
patent: WO 02/062475 (2002-08-01), None
patent: WO 2005/028710 (2005-03-01), None
Walker, Kathleen L., et al., “Off-Line Coupling of Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry”. Analytical Chemistry, vol. 67, No. 22, pp. 4197-4204, 1995.
Hage, David S., “Survey of Recent Advances in Analytical Applications of Immunoaffinity Chromatography”, Journal of Chromatography B, vol. 715, pp. 3-28, 1998.
Hage, David S., “Affinity Chromatography: A Review of Clinical Applications”, Clinical Chemistry, vol. 45, No. 5, pp. 593-615, 1999.
Muronetz, Vladimir I., et al., “Use of Protein-Protein Interactions in Affinity Chromatography”, Journal of Biochemical and Biophysical Methods, vol. 49, pp. 29-47, 2001.
Baczek, Tomasz., et al., “Quantitative Structure/Retention Relationships in Affinity Chromatography”, Journal of Biochemical and Biophysical Methods, vol. 49, pp. 83-98, 2001.
Burgess, Richard R., et al., “Advances in Gentle Immunoaffinity Chromatography”, Protein Technologies and Commercial Enzymes, pp. 304-308.
Hage, David S., et al., “Characterization of the Protein Binding of Chiral Drugs by High-Performance Affinity Chromatography Interactions of R- and S-Ibuprofen with Human Serum Albumin”, Journal of Chromatography A., vol. 693, pp. 23-32, 1995.
Hofstetter, Heike, et al., “Enantiomer Separation Using BSA as Chiral Stationary Phase in Affinity OTEC and OTLC”, Journal of Microcolumn Separations, vol. 10. No. 3, pp. 287-291, 1998.
Hofstetter, Oliver, et al., “Direct Resolution of Enantiomers in High-Performance Immunoaffinity Chromatography Under Isocratic Conditions”, Analytical Chemistry, vol. 74, No. 9, pp. 2119-2125, 2002.
Fitos, I., et al., “Species-Dependency in Chiral-Drug Recognition of Serum Albumin Studied by Chromatographic Methods”, Journal of Biochemical and Biophysical Methods, vol. 54, pp. 71-84, 2002.
Hsieh, Frank Y.L., et al., “Automated Analytical System for the Examination of Protein Primary Structure”, Analytical Chemistry, vol. 68, No. 3, pp. 455-462, 1996.
Wang, Can, et al., “Integration of Immobilized Trypsin Bead Beds for Protein Digestion Within a Microfluidic Chip Incorporating Capillary Electrophoresis Separations and an Electrospray Mass Spectrometry Interface”, Rapid Communications in Mass Spectrometry, vol. 14, pp. 1377-1383, 2000.
Wang, Shihong, et al., “Proteolysis of Whole Cell Extracts with Immobilized Enzyme Columns as Part of Multidimensional Chromatography”, Journal of Chromatography A, vol. 913, pp. 429-436, 2001.
Peterson, Dominic S., et al., “High-Throughput Peptide Mass Mapping Using a Microdevice Containing Trypsin Immobilized on a Porous Polymer Monolith Coupled to MALDI TOF and ESI TOF Mass Spectrometers”, Journal of Proteome Research, vol. 1, pp. 563-568, 2002.
Peterson, Dominic S., et al., “Enzymatic Microreactor-on-a-Chip: Protein Mapping Using Trypsin Immobilized on Porous Polymer Monoliths Molded in Channels of Microfluidic Devices”, Analytical Chemistry, vol. 74, No. 16, pp. 4081-4088, 2002.
Slysz, Gordon, W., et al., “On-Column Digestion of Proteins in Aqueous-Organic Solvents”, Rapid Communications in Mass Spectrometry, vol. 17, pp. 1044-1050, 2003.
Zhang, Boyan, et al., “Frontal Affinity Chromatography Coupled to Mass Spectrometry for Screening Mixtures of Enzyme Inhibitors”, Analytical Biochemistry, vol. 299, pp. 173-182, 2001.
Baynham, Michael T., et al., “Multidimensional On-Line Screening for Ligands to the α3β4 Neuronal Nicotinic Acetylcholine Receptor Using an Immobilized Nicotinic Receptor Liquid Chromatographic Stationary Phase”, Journal of Chromatography B, vol. 772, pp. 155-161, 2002.
Moaddel, Ruin, et al., “Immobilized Receptor-and Transporter-Based Liquid Chromatographic Phases for On-Line Pharmacological and Biochemical Studies: a Mini-Review”, Journal of Chromatography B, vol. 768, pp. 41-53, 2002.
Moaddel, Ruin, et al., “Multiple Receptor Liquid Chromatographic Stationary Phases: The Co-Immobilization of Nicotinic Receptors, γ-Amino-Butyric Acid Receptors, and N-Methyl D-Aspartate Receptors”, Pharmaceutical Research. vol. 19, No. 1, pp. 104-107, 2002.
Moaddel, Ruin, et al., “Immobilized Nicotinic Receptor Stationary Phases: Going with the Flow in High-Throughput Screening and Pharmacological Studies”, Journal of Pharmaceutical and Biomedical Analysis, vol. 30, pp. 1715-1724, 2003.
Hodgson, Richard J., et al., “Capillary-Scale Monolithic Immunoaffinity Columns for Immunoextraction with In-Line Laser-Induced Fluorescence Detection”, Analytical Chemistry, vol. 77, No. 14, pp. 4404-4412, 2005.
Hodgson, Richard J., et al., “Protein-Doped Monolithic Silica Columns for Capillary Liquid Chromatography Prepared by the Sol-Gel Method: Applications to Frontal Affinity Chromatography”, Analytical Chemistry, vol. 76, No. 10, pp. 2780-2790, 2004.
Annesley, Thomas M., “Ion Suppression in Mass Spectrometry”, Clinical Chemistry vol. 49, No. 7, pp. 1041-1044, 2003.
Zuberovic, Aida, et al., “Capillary Electrophoresis Off-Line Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry in Intact and Digested Proteins Using Cationic-Coated Capillaries”, Rapid Communications in Mass Spectrometry, vol. 18, pp. 2946-2952, 2004.
Tegeler, Tony, J., et al., “Microdeposition Device Interfacing Capillary Electrochromatography and Microcolumn Liquid Chromatography with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry”, Analytical Chemistry, vol. 76, No. 22, pp. 6698-6706, 2004.
Weinmann, Wolfgang, et al., “Capillary Electrophoresis-Matrix-Assisted Laser-Desorption Ionization Mass Spectrometry of Proteins”, Journal of Chromatography A, vol. 680, pp. 353-361, 1994.
Zhang, Haiying, et al., “Capillary Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry; Continuous Sample Deposition on a Matrix-Precoated Membrane Target”, Journal of Mass Spectrometry, vol. 31, pp. 1039-1046, 1996.
Zhang, Haying, et al., “Combining Solid-Phase Preconcentration, Capillary Electrophoresis and Off-Line Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Intracerebral Metabolic Processing of Peptide E In Vivo”, Journal of Mass Spectrometry, vol. 34, pp. 377-383, 1999.
Bogan, Michael J., et al., “Preliminary Investigation of Electrodynamic Charged Droplet Processing to Couple Capillary Liquid Chromatography with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry”, Rapid Communications in Mass Spectrometry, vol. 18, pp. 2673-2681, 2004.
Zhang, Boyan, et al., “Combining Liquid Chromatography with MALDI Mass Spectrometry Using a Heated Droplet Interface”, Analytical Chemistry, vol. 76, No. 4, pp. 992-1001, 2004.
Miliotis, Tasso, et al., “Capillary Liquid Chromatography Interfaced to Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mas
Brennan John D.
Covey Tom R.
Davidson William R.
Kovarik Peter
Shushan Bori
Bereskin & Parr
Berman Jack
Folkins Patricia
Hashmi Zia R.
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