Frog embryo and egg-derived tumor cell anti-proliferation protei

Details Frog embryo and egg-derived tumor cell anti-proliferation protei Frog embryo and egg-derived tumor cell anti-proliferation protei

1994-08-01

1996-09-24

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C07K 14475

Patent

active

055592120

ABSTRACT:
Rana pipiens eggs are subjected to fertilization and the embryos are grown to a predetermined stage of development. The embryos and unfertilized eggs are then subjected to mechanical processing in the presence of a weakly acidic buffer to produce an extract. The extract is subjected to ion-exchange chromatography and size-exclusion chromatography.
The resulting pharmaceutical has activity against certain cancer cells. The amino acid sequence and composition of the pharmaceutical are disclosed.

REFERENCES:
patent: 4289690 (1981-09-01), Pertha et al.
patent: 4882421 (1989-11-01), Shogen et al.
Shibaya et al. 1989, Biosis Number: 89101888 of Development 106(4): 799-808.
Shibiya et al. 1988. File 155 Accession No. 88313378 of Dev Biol. 129(1): 253-264.
Sigma Chemical Co. Price List, 1988, St. Louis, No. p. 1023.
Hird et al. 1990. Genes and Cancer, Corney et al (eds.), John Wiley & Sons Ltd., NY, pp. 183-189.
Ardelt et al. 1991. J. Biol Chem. 266(1): 245-251.

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