Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2009-11-17
2011-11-08
Minnifield, Nita M (Department: 1645)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C530S300000, C530S350000, C530S324000, C530S326000, C530S325000, C435S007100, C435S252700, C435S069100, C436S546000
Reexamination Certificate
active
08053209
ABSTRACT:
The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
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Aoki Kei Roger
Fernandez-Salas Ester
Steward Lance E.
Abel Kenton
Allergan Inc.
Condino Debra
Minnifield Nita M
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