Frequency domain fluorometry using coherent sampling

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2504581, 356317, G06G 758, G01N 2164

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active

051518694

ABSTRACT:
Apparatus and methods for measuring response of a chemical composition to light, as by frequency domain, phase angle, or modulation fluorometry and/or phosphorimetry. Pulsed light having a known fundamental frequency is applied to the composition thereby eliciting response light varying at the fundamental frequency and at harmonics thereof. The response light signal may be sampled according to a wave-skipping sampling scheme, and the sampled response signal may be transformed into a frequency domain representation, as by a digital fast Fourier transform technique. A reference signal derived from the excitation light may be similarly processed and the results used as a basis for comparison to derive properties of the chemical composition such as phase and modulation response of the composition to the fundamental and harmonic frequencies. The invention provides rapid monitoring and determination of luminescence characteristics. Multiple excitation frequencies may be applied simultaneously and the resulting response signals may be separated and separately sampled.

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