Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1997-12-24
2000-06-20
MacMillan, Keith D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530417, 5303871, 436828, 436161, 210656, 210635, B01D 6100, A23J 100, C07K 1600
Patent
active
060779403
ABSTRACT:
The present invention is directed to novel methods for enhancing the ability to separate a species of interest from other different species present in a free solution mixture thereof which takes advantage of interactions that occur between soluble, small molecular weight ligands and the species of interest. The small molecular weight ligands employed in the present invention function to interact with a species of interest in a mixture of different species through either affinity, hydrophobic and/or ionic interactions, thereby altering the molecular weight, hydrodynamic volume and/or isoelectric point of the species of interest and rendering it separable from other component(s) in the mixture.
REFERENCES:
patent: 4762617 (1988-08-01), Stevens et al.
patent: 4780210 (1988-10-01), Hsia
patent: 4960702 (1990-10-01), Rice et al.
patent: 5053334 (1991-10-01), Arathoon et al.
patent: 5356637 (1994-10-01), Loosen et al.
patent: 5490937 (1996-02-01), van Reis
patent: 5759404 (1998-06-01), Ericsson et al.
van Eijndhoven, R.H.C.M., et al., "Protein Fractionation Using Electrostatic Interactions in Membrane Filtration", Biotechnology and Bioengineering, vol. 48, pp:406-414 (1995).
Dong, X.Y., et al., "Multistage affinity cross-flow filtration: mathematical modelling and analysis", Bioprocess Engineering, vol. 15, pp. 99-104 (1996).
Flaschel, E., et al., "Ultrafiltration for the Separation of Biocatalysis", Advances in Biochemical Engineering/Biotechnology, vol. 26, pp. 123-140 (1983).
Ling, T.G.I., et al., "Membrane Filtration Affinity Purification (MFAP) of Dehydrogenases Using Cibacron Blue", Biotechnology and Bioengineering, vol. 24, pp. 1321-1325 (1989).
Nguyen, A.L., et al., "Synthesis and Applications of Water-Soluble Reactive Polymers for Purification and Immobilization of Biomolecules", Biotechnology and Bioengineering, vol. 34, pp. 1186-1190 (1989).
Janson, Jan-Christer, et al., "A Bacterial Dextranase", Enzymes of Complex Saccharide Utilization, vol. 105: pp. 615-621 (1966).
Bartling, G., et al., "Separation of Trypsin and Peroxidase by Ultrafiltration Using Crosslinked Soybean Trypsin Inhibitor", Biotechnology and Bioengineering, vol. XVII, pp. 1023-1027 (1976).
Mattiasson, B., et al., "Isolation of Concanavalin A From Seeds of Conavalia Ensiformis", Journal of Chromatography, vol. 283, pp. 323-330 (1984).
Weiner, C., et al., "Affinity Cross-Flow Filtration: Purification of IgG with a Novel Protein A Affinity Matrix Prepared from Two-Dimensional Protein Crystals", Biotechnology and Engineering, vol. 44, pp. 55-65 (1994).
Ghosh, R., et al., "Modeling and Simulation of the Washing Phase of an Affinity Ultrafiltration Sysetm", Separation Science and Technology, vol. 31(1) pp. 125-131 (1996).
Luong, J.H.T., et al., "Synthesis and Characterization of a Water-Soluble Affinity Polymer for Trypsin Purification", Biotechnology and Bioengineering, vol. 31, pp. 439-446 (1988).
Luong, J.H.T., et al., "A Continuous Affinity Ultrafiltration Process for Trypsin Purification", Biotechnology and Bioengineering, vol. 31, pp. 516-520 (1988).
Mattiasson, B., et al., "Ultrafiltration Affinity Purification, A Process for Large-Scale Biospecific Separations", Ultrafiltration Affinity Purification, pp. 99-114, (1989).
Male, K.B., et al., "Isolation of Urokinase by Affinity ultrafiltration", Biotechnoology and Bioengineering, vol. 35, pp. 87-93 (1990).
Adamski-Medda, D., et al., "Biospecific Ultrafiltration: A Promising Purification Technique for Proteins", Journal of Membrane Science, vol. 9, pp. 337-342 (1981).
Mattiasson, B., et al., "Ultrafiltration Affinity Purification", Annals New York Academy of Science, vol. 413, pp. 307-309 (1983).
Conley Deirdre L.
Genentech Inc.
MacMillan Keith D.
Ponnaluri P.
LandOfFree
Free solution ligand interaction molecular separation method does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Free solution ligand interaction molecular separation method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Free solution ligand interaction molecular separation method will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1854540