Free-flow electrophoretic separation method and apparatus theref

Details Free-flow electrophoretic separation method and apparatus theref Free-flow electrophoretic separation method and apparatus theref

1987-03-02

1988-06-07

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041801, 2041832, 2041833, 2041826, 204299R, 204301, G01N 2728, G01N 2726

Patent

active

047494581

ABSTRACT:
The invention discloses a free-flow electrophoretic separation method and apparatus for separating charged substances such as proteins, cells, and the like, by electrophoresis, which comprises supplying a mixed solution containing the charged substances to be separated, which are dissolved in a separation buffer, into a separation chamber, circulating the mixed solution inside the separation chamber, applying a d.c. voltage to the mixed solution to cause the electrophoresis of the charged substances and to separate them, and withdrawing the charged substances thus separated from outlets disposed at one and the other end of the separation chamber.

REFERENCES:
patent: 2878178 (1959-03-01), Bier
patent: 3989613 (1976-11-01), Gritzner
patent: 4155831 (1979-05-01), Bhattacharya
patent: 4172774 (1979-10-01), Moeglich
patent: 4465583 (1984-08-01), Lovegrove

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