Free cholesterol analogs bearing a boron dipyrromethene...

Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...

Reexamination Certificate

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Reexamination Certificate

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08030482

ABSTRACT:
The present invention relates to free cholesterol analogs bearing a boron dipyrromethene difluoro (BODIPY) fluorophore in the side chain and methods of preparation. The compounds of the present invention can be used in fluorescence spectroscopy and fluorescence microscopy to visualize the exchange, distribution, and trafficking of free cholesterol between living cells and to monitor the movement of free cholesterol between ordered and disordered lipid domains in membranes.

REFERENCES:
Shaw et al., “Correlated Fluorescence-Atomic Force Microscopy of Membrane Domains: Structure of Fluorescence Probes Determines Lipid Localization.” Biophysical Journal, vol. 90, pp. 2170-2178, Mar. 2006.
Li et al., “First Synthesis of Free Cholesterol—BODIPY Conjugates,”J. Org. Chem., 71, 1718-1721 (2006).
Shaw et al., “Correlated Fluorescence-Atomic Force Microscopy of Membrane Domains: Structure of Fluorescence Probes Determines Lipid Localization,”Biophysical Journal, 90, 2170-2178 (2006).
Shaw, et al., “Tracking peptide-membrane interactions: Insights from in situ coupled confocal-atomic force microscopy imaging of NAP-22 peptide insertion and assembly,”Journal of Structural Biology, 155, 458-469 (2006).

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