Frame-shifting PCR for germline immunoglobulin genes...

Details Frame-shifting PCR for germline immunoglobulin genes... Frame-shifting PCR for germline immunoglobulin genes...

2007-07-10

2007-07-10

Fredman, Jeffrey (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S070100

Reexamination Certificate

active

10880238

ABSTRACT:
A method for preparing an antigen-specific antibody by constructing a library of phage-displayed single chain variable fragment of an antibody with a novel frame-shifting PCR is disclosed. Also disclosed is a method for preparing a clone for producing an antigen-specific antibody.

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patent: 2004/0081638 (2004-04-01), Kyle
Wagle et al., CD19 regulates B cell antigen receptor-mediated MHC class II antigen processing. Vaccine (2000) 18: 376-386.
Buck et al., Design strategies and performance of custom DNA sequencing primers. BioTechniques (1999) 27: 528-536.

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