Fragments of fluorescent proteins for protein fragment...

Details Fragments of fluorescent proteins for protein fragment... Fragments of fluorescent proteins for protein fragment...

2007-01-23

2007-01-23

Brusca, John S. (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C530S350000

Reexamination Certificate

active

10724178

ABSTRACT:
The present invention is directed to Protein-fragment Complementation Assays (PCAs) and assay compositions based on fluorescent proteins. The invention provides methods for fragmenting fluorescent proteins and generating mutant fragments with desired spectral characteristics for PCA. The invention encompasses assays and compositions based on fluorescent proteins from the species Aequorea, Anemonia and Anthozoa. In particular, the invention is directed to fragments of mutant fluorescent proteins having improved spectral properties over the wild-type proteins. The invention encompasses fragments of mutant versions ofA. Victoriagreen fluorescent protein (GFP), in particular yellow fluorescent proteins (EYFP and super-EYFP), ‘Venus’, cyan, ‘citrine’, blue, cyan-green, and photoactivatable variants of GFP.

REFERENCES:
patent: 6270964 (2001-08-01), Michnick et al.
patent: 6294330 (2001-09-01), Michnick et al.
patent: 6428951 (2002-08-01), Michnick et al.
Ghosh et al. Antiparallel Leucine Zipper-Directed Protein Reassembly: Application to the Green Fluorescent Protein. J. Am. Chem Soc. vol. 122, pp. 5658-5659 (2000).

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