Fractionation of whey proteins by complex formation

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin

Reexamination Certificate

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C530S344000, C424S535000

Reexamination Certificate

active

06900290

ABSTRACT:
Disclosed is a method of fractionating α-lactalbumin from β-lactoglobulin in whey or whey protein concentrate. The method includes the steps of contacting whey or whey protein concentrate with a complexing agent so that the complexing agent forms insoluble complexes with β-lactoglobulin present in the whey or whey protein concentrate. The insoluble complexes are then separated from the whey or whey protein concentrate, thus yielding a precipitate that is predominately β-lactoglobulin and is substantially devoid of α-lactalbumin, and a supernatant that predominately α-lactalbumin and is substantially devoid of β-lactoglobulin.

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patent: 5925737 (1999-07-01), Tomasula et al.
patent: 5986063 (1999-11-01), Etzel
patent: 0 340 035 (1989-11-01), None
da Fonseca LM, Bradley RL. Fractionation of whey proteins by complex formation and membrane filtration. University of Wisconsin. Dissertation Abstracts International, (1999) vol. 61, No. 2. Particularly pp. 105-178.*
Eigel et al. (1984)J. Dairy Sci. 67:1599-1631.
Fonseca et al. (1999), Fractionation of whey proteins by complex formation (Abstract),Journal of Dairy Science, vol. 82, Suppl. 1, p. 27.
Mott et al., (1999), Effect of Xanthan Gum on Enhancing the Foaming Properties of Whey Protein Isolate,JAOCS, vol. 76, No. 11, pp. 1383-1386.
Pearce & Kinsella (1978)J. Agric. Food Chem26:716-723.

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