Fractionation of sperm

Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis

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195 18, A61K 3552, C12K 900

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active

040092607

ABSTRACT:
Motile sperm are separated from immotile and abnormal sperm of mammalian semen by maintaining, at a temperature at which the motile sperm are motile, a layer of at least the sperm portion of the semen in interfacial contact with a discrete lower layer of an aqueous contacting medium physiologically acceptable to the sperm in which the motile sperm have a lower migration rate than in the upper layer, until the motile sperm of the semen have migrated at a preferential rate to the contacting medium, thereby providing a sperm fraction consisting essentially of normal motile sperm with enhanced Y-sperm content. Repeating the process with a second contacting medium physiologically acceptable to the sperm which further retards the migration rate of the motile sperm increases substantially the Y- to X-sperm ratio of the sperms which migrate thereto. The resulting sperm fraction, because of its enhanced Y-sperm content, can be used for artificial insemination to increase the incidence of male offspring. The first step alone can be used to improve the quality of semen having an abnormally low normal motile sperm content, thereby increasing the chances of a successful insemination.

REFERENCES:
patent: 3687806 (1972-08-01), Bovenkamp
S.W.B. Research Corp. --Chem. Abst., vol. 66 (1967) p. 44818q.
Shilling--J. Reprod. Fert., vol. 11 (1966) pp. 469-472.
Ericsson et al.--Nature, vol. 246, Dec. 1973 pp. 421-424.
Population Reports--Series I, No. 2 (May, 1975) pp. 25, 29 and 31.

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