Fractionation of macro-molecules using asymmetric pulsed...

Details Fractionation of macro-molecules using asymmetric pulsed... Fractionation of macro-molecules using asymmetric pulsed...

2005-04-19

2005-04-19

Warden, Jill (Department: 1743)

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

C422S050000, C422S052000, C422S051000, C422S063000, C422S068100, C422S081000, C422S082000, C422S082050, C422S082080, C422S082090, C422S105000, C422S105000, C422S105000, C422S105000, C436S008000, C436S043000, C436S174000, C204S600000, C204S601000, C204S450000, C204S451000, C204S458000, C700S001000, C700S266000, C700S273000

Reexamination Certificate

active

06881317

ABSTRACT:
A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

REFERENCES:
patent: 4473452 (1984-09-01), Cantor et al.
patent: 4523320 (1985-06-01), Stappaerts
patent: 4693804 (1987-09-01), Serwer
patent: 4737251 (1988-04-01), Carle et al.
patent: 4740283 (1988-04-01), Laas et al.
patent: 4830726 (1989-05-01), Stamato et al.
patent: 5011586 (1991-04-01), Finney et al.
patent: 5106468 (1992-04-01), Chimenti
patent: 5116471 (1992-05-01), Chien et al.
patent: 5122248 (1992-06-01), Karger et al.
patent: 5178737 (1993-01-01), Lai
patent: 5405519 (1995-04-01), Schwartz
patent: 5427663 (1995-06-01), Austin et al.
patent: 5972190 (1999-10-01), Richman
patent: 6027623 (2000-02-01), Ohkawa
patent: 6110339 (2000-08-01), Yager et al.
patent: 6156273 (2000-12-01), Regnier et al.
patent: 6176990 (2001-01-01), Yager et al.
patent: 6254754 (2001-07-01), Ross et al.
patent: 6280590 (2001-08-01), Cheng et al.
patent: 6596144 (2003-07-01), Regnier et al.
patent: 0560974 (1997-01-01), None

Affiliated with

Also associated with

Rating

Fractionation of macro-molecules using asymmetric pulsed... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fractionation of macro-molecules using asymmetric pulsed..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fractionation of macro-molecules using asymmetric pulsed... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3373818