Fractionation method for nucleotide fragments

Details Fractionation method for nucleotide fragments Fractionation method for nucleotide fragments

1996-11-15

1998-10-06

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 6, 435 912, 536 231, 536 243, C12Q 168, C12Q 170, C07H 2104, C12P 1934

Patent

active

058174648

ABSTRACT:
A fractionation method for DNA fragments according to the present invention comprises a first step of preparing a probe chip or a set of probe chips immobilizing independently a DNA probe having a first sequence part having a specific known sequence part together with a part of enzyme recognition sequence and a second sequence part composed of a combination of one to six bases adjacent to the first sequence part at 3' terminus, a second step of introducing a DNA oligomer composed of a part of enzyme recognition sequence and a sequence complementary to the known sequence part into the fragment termini of DNA fragments from restriction enzyme cleavage, and a third step of placing the probe chip or the set of probe chips in a solution containing the nucleotide fragments with the introduced DNA oligomer produced at the second step, for at least hybridization and the complementary strand extension of the DNA probe, whereby the DNA fragments are fractionated.

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