Fowl cholera vaccine and its preparation

Details Fowl cholera vaccine and its preparation Fowl cholera vaccine and its preparation

1978-08-21

1979-10-02

Rose, Shep K.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

A61K 3902

Patent

active

041698860

ABSTRACT:
The present invention relates to a live vaccine against Pasteurella multocida, for application of poultry by injection, per os or as aerosol, containing an attenuated non-virulent genetically stable strain of Pasteurella multocida, to a process for producing an attenuated genetically stable non-virulent strain of Pasteurella multocida, which comprises cultivating a virulent field strain, selecting after 18 hours at 37.degree. C. colonies of uniform size, of about 4 to 5 mm diameter, suspending these in a pH 5.5 buffer, subjecting same to a mutagen at a concentration causing a mortality of 90-99% after 30 minutes at 37.degree. C., centrifuging the microorganisms, washing same, plating same and selecting colonies of not more than 2.5 to 3 mm diameter after 18 hours at 37.degree. C., purifying by reisolation of single colonies, testing for antigenic specificity and virulence, and selecting avirulent genetically stable immunologically effective mutants for use in vaccines, and to a process for the vaccination of poultry which comprises applying such a vaccine by injection, per os or as aerosol.

REFERENCES:
heddleston et al., Poultry Science, 54 (1): 217-221, Jan. 1975, "Fowl Cholera: Immunologic and Serologic Response in Turkeys to Live Pasteurella multocida Vaccine Administered in the Drinking Water".
Sulong et al., Veterinary Microbiology, 1 (1): 3-14 (1976), "Studies on Pasteurella multocida IV, Immunoflurescence Detection of the Organisms in Spleen and Lungs of Turkeys Vaccinated with Live Oral Vaccines".
Coates et al., Poultry Science, 56 (1): 273-276 (1977), "The Response of Turkeys to Varying Doses of Live Oral Pasteurella multocida Vaccine".
Bierer, Poultry Science 56 (1): 327-330 (1977), "An Evaluation of the Effect of Various Concentrations of the Clemson University Pasteurella multocida Drinking Water Vaccine on the Immune Response Against Fowl Cholera Disease in Turkeys".

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