Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2011-04-12
2011-04-12
Epps-Smith, Janet L. (Department: 1633)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S029000, C435S032000
Reexamination Certificate
active
07923204
ABSTRACT:
Disclosed herein is a novel forward mutation assay based on 5-fluorouracil (5-FU) resistance which utilizes a strain ofSalmonella typhimuriumderived from the Ames strain TA100. More specifically, the invention provides a high throughput alternative to the standard Ames mutation assay for the evaluation of the genotoxicity activity of compounds during an early stage of the drug development process. The invention also identifies the upp locus as a mutational target that is capable of detecting a diverse spectrum of mutagenic events and further describes aS. typhimuriumtester strain, designated FU100 (his+, rfa, ΔuvrB, pkM101, 5-fluorouridine resistant) for use in the assay of the invention.
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Glaab Warren E.
Miller Judith E.
Skopek Thomas R.
Vlasakova Katerina
Epps-Smith Janet L.
Heber Sheldon O.
Merck Sharp & Dohme Corp.
Wenk Melissa B.
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