Formulations for fluorogenic peroxidase assays

Details Formulations for fluorogenic peroxidase assays Formulations for fluorogenic peroxidase assays

1998-12-08

2000-03-21

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435 71, 436172, C12Q 128, C12Q 100, G01N 3353, G01N 2176

Patent

active

060401508

ABSTRACT:
An improved method is disclosed for assaying peroxidase or peroxide activity utilizing a substrate solution containing a fluorogenic phenolic compound, hydrogen peroxide and a metal chelating compound. The improvement inclosed including within the assay, in an amount sufficient to enhance the effective working range of the assay, a boron acid or salt thereof, or a phosphine-based or hydride-based reducing agent.

REFERENCES:
patent: 4152411 (1979-05-01), Schall, Jr.
patent: 5543332 (1996-08-01), Lihme et al.
Zaitsu et al. Analytical Biochemistry 109:109-113, 1980.

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