Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Reexamination Certificate
2007-06-04
2010-06-29
Burkhart, Michael (Department: 1633)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
C435S245000, C435S006120, C435S029000, C435S471000
Reexamination Certificate
active
07745200
ABSTRACT:
The present invention provides formaldehyde dehydrogenase genes (FLD) from methylotrophic yeasts. The FLD structural genes confer resistance to formaldehyde and are therefore useful as a selectable marker in methylotrophic yeasts. The FLD promoter sequences are strongly and independently induced by either methanol as sole carbon source (with ammonium sulfate as nitrogen source) or methylamine as sole nitrogen source (with glucose as carbon source). Induction under either methanol, methylamine or both provides levels of heterologous gene expression comparable to those obtained with the commonly used alcohol oxidase I gene promoter (PAOX1). The FLD promoter ofPichia pastoris(PFLD1) is an attractive alternative to PAOX1for expression of foreign genes inP. pastoris, allowing regulation by carbon (methanol) or nitrogen (methylamine) source within the same expression strain. Yeast strains, expression cassettes, expression vectors, and host cells-comprising an FLD gene promoter and 3′ termination sequence are also provided.
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Burkhart Michael
Research Corporation Technologies Inc.
Scully , Scott, Murphy & Presser, P.C.
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