Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-05-13
1996-07-02
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436 92, 436161, G01N 3353, G01N 3300
Patent
active
055321313
ABSTRACT:
New and improved methods for collecting neonatal meconium samples, preparing meconium specimens for testing and for chemically analyzing neonatal meconium samples to determine their chemical composition are provided. A novel extraction method is employed in accordance with the invention to provide a non-aqueous, concentrated "cocktail" meconium extract containing substantially all of many possible target analytes in a single extraction step. Preliminary screening by fluorescence polarization immunoassay methods may be performed on the cocktail extract to qualitatively determine the presence of the target analytes in the meconium sample. If a positive preliminary result is obtained, new and improved quantitative GC/MS confirmatory procedures are provided by this invention to unequivocally identify and quantitate the amount of target analyte present in the sample in terms of nanograms of analyte per gram of meconium tested.
REFERENCES:
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patent: 5185267 (1993-02-01), Ostrea, Jr.
Murphey et al., Quantitation of benzoylnorcgonine and other cocaine metabolites in meconium by high-performance liquid chromatography, J. Chromatog. 613:330-335, 1993.
Clark et al., "Analysis of Cocaine and Benzoylecgonine in Meconium of Infants Born to Cocaine Dependent Mothers," Clinical Chemistry, Jul., 1990, vol. 36, No. 6, Abstract 0327, p. 1022.
Rosenzweig et al., "Neonatal Drugs of Abuse Screening in Meconium, A Comparison Between Abbott TDx and Syva ETZ," Clinical Chemistry, Jul., 1990, vol. 36, No. 6, Abstract 0334, p. 1023.
Jennison, "An Evaluation of Clean Screen Extraction Columns for the Analysis of Benzoylecgonine," Clinical Chemistry, Jul., 1990, vol. 36, No. 6, Abstract 0335, p. 1023.
Clark et al., "The Analysis of Cocaine and Benzoylecgonine in Meconium," Journal of Analytical Toxicology, Jul./Aug. 1992, vol. 16, pp. 261-263.
Lewis Douglas Edward
Mosher Mary E.
Wortman Donna C.
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