Chemistry: molecular biology and microbiology – Vector – per se
Patent
1994-03-31
1995-10-17
Martinell, James
Chemistry: molecular biology and microbiology
Vector, per se
4351723, 4351731, 4351736, 4352523, C12N 1500, C12N 1563
Patent
active
054590728
ABSTRACT:
The present invention is directed to integration vectors including food-grade integration vectors, a method of increasing the stability of the inheritance of genes in microorganisms and transformed microorganisms containing an integrated vector and which exhibit an increase in the stability of inheritance of a gene. The integration vector is comprised of a first DNA sequence encoding a temperature sensitive plasmid maintenance and replication region, a second DNA sequence encoding at least one selectable marker gene, a third DNA sequence encoding an insertion sequence, and a fourth DNA sequence which encodes an unstable gene. The method of increasing the stability of the inheritance of a gene includes introducing the integration vector into a host microorganism, selecting transformed microorganisms, and confirming that selected microorganisms exhibit an increase in the stability of inheritance of a gene encoding a trait. Transformed microorganisms are provided which have at least one integration vector integrated into the genome and which exhibit an increase in the stability of the inheritance of a gene encoding a trait. The invention also provides DNA sequences substantially corresponding to a temperature sensitive plasmid maintenance and replication sequence, a nisin resistance gene, and a novel lactococcal insertion sequence.
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McKay Larry L.
Polzin Kayla M.
Martinell James
Regents of the University of Minnesota
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