Folded recombinant catalytic fragments of multidomain serine...

Details Folded recombinant catalytic fragments of multidomain serine... Folded recombinant catalytic fragments of multidomain serine...

2003-08-08

2010-02-23

Desai, Anand U (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S226000, C435S252300, C435S252330, C435S320100, C536S023400

Reexamination Certificate

active

07666627

ABSTRACT:
The invention relates to unglycosylated folded C-terminal fragments of a multidomain serine protease of the complement cascade obtainable by expression in a bacterial host, wherein said serine protease is capable of binding a recognition molecule of the complement cascade, e.g. C1 or MBL. The invention also relates to methods and bacterial expression vectors for the preparation of said fragments, uses of said fragments for raising antibodies and screening substrates or inhibitors of said serine proteases and uses of the fragments in research and treatment of complement related disorders. The invention also relates to assay methods for assessing MASP-1 and MASP-2 levels in a sample of biological origin.The invention provides for research tools, assays and diagnostic kits useful in complement research and research and diagnosis of complement related disorders.

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