Foam flotation process for separating Bacillus thuringiensis spo

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435832, C12N 300

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active

042476445

ABSTRACT:
Crystal-enriched suspensions are derived from sporulated cultures of Bacillus thuringiensis by a foam flotation process in which added gelatin causes the spores to be selectively entrained in the foam and thereby separated from the suspensions.

REFERENCES:
patent: 3075887 (1963-01-01), Silliker et al.
patent: 3087865 (1963-04-01), Drake et al.
Truskov "Isolating Pure Inclusion Produced in Cultures of Bacillus Thuringiensis", Cited in Chemical Abstracts, vol. 72 (1970), Abstract No. 77484e.
Kirk-Othmer, "Encyclopedia of Chemical Technology", Interscience Publishers, vol. 9, Second Edition, 1969, pp. 884 and 893-896.
Angus, "Separation of Bacterial Spores & Parasporal Bodies with a Fluorocarbon", J. Insect. Path., vol. 1, pp. 97-98 (1959).
Bateson, "Isolation of Crystalline Parasporal Bodies of Bacillus thuringiensis", Nature, vol. 205 (1965), pp. 622-623.
Pendelton, "Separation of the Spores & Crystals of B. thuringiensis", Nature, vol. 212 (1966), pp. 180-184.
Gingrich", "Flotation Procedure for Producing Spore Free Crystals", J. Invertebr. Path, vol. 10, (1968), pp. 180-184).
Fast., "The S-Endotoxin of B. thuringiensis III Rapid Method for Separating Parasporal Bodies from Spores", J. Inverteb Path, vol. 20 (1972), pp. 139-140.
Sharpe et al., "Separation of Spores & Parasporal Crystals of B. thuringiensis in Gradients of Certain X-ray Contrast agents", Appl. Environ. Microbiol., vol. 30 (1975), pp. 1052-1053.
Milore et al., "B. thuringiensis S. Endotoxin: Improved Technique for Separating Crystals from Spores", J. Invertebr. Path, vol. 29 (1977), pp. 230-231.

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