Fluorophores for encapsulation into liposomes

Details Fluorophores for encapsulation into liposomes Fluorophores for encapsulation into liposomes

1989-07-24

1990-11-13

Griffin, Ronald W.

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

536 53, 424 71, 424 9, 544300, 549223, 549403, 558 8, C07D31182, C07D47308, A61K 4300

Patent

active

049700740

ABSTRACT:
The present invention provides novel fluorescein derivative compounds having fluorescence spectrum and quantum yield characteristics similar to those of fluorescein. The compounds are readily synthesized and purified and are readily soluble in water at self-quenching concentrations. Significantly, due to the presence of polar polyhydroxy group substituents and the absence of metal-chelating groups, these fluorescein derivatives are susceptible to minimal leakage across liposome membranes and have fluorescence characteristics minimally sensitive to the presence of metal ions. Compounds of the invention are thus exceptionally suitable for use in the development of highly storage stable liposome preparations to be employed in immunolytic assays involving human body fluid samples.

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