Fluorophore assisted derivatization analysis of carbohydrates

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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536127, C12Q 134

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active

060487070

ABSTRACT:
Methods and kits for structurally analyzing carbohydrate molecules are taught. Carbohydrates for analysis are derivatized (preferably methylated) and then hydrolyzed into constituent monosaccharides. The derivatized monosaccharides are then labeled by a fluorophore and separated from one another by electrophoresis. The identity of derivatized monosaccharides is established by comparison with identification standards. The electrophoresis separation patterns may be visualized by a charged coupled device camera or photographically.

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