Fluorophore-assisted cloning analysis

Details Fluorophore-assisted cloning analysis Fluorophore-assisted cloning analysis

1992-02-25

1993-11-02

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 74, 536 111, C12N 1510

Patent

active

052582957

ABSTRACT:
Various assays for the detection and quantitation of carbohydrate-interacting proteins are disclosed. Carbohydrate-interacting proteins include carbohydrate modifying enzymes and carbohydrate binding proteins. The assays employ a fluorophore-labelled carbohydrate substrate that either is capable of being structurally modified by the carbohydrate-modifying enzyme being assayed or changes its electrophoretic gel migration tube upon interaction with a carbohydrate-binding protein. Methods for cloning genes encoding carbohydrate-modifying enzymes are also disclosed.

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