Fluorophore assisted carbohydrate electrophoresis diagnosis

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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Details

204299R, 2041801, G01N 2726, G01N 27447

Patent

active

052059170

ABSTRACT:
The subject invention provides methods and kits for conveniently diagnosing various physiological conditions that produce altered levels of specific carbohydrates, known as diagnostic carbohydrates. Measurement of the levels of the diagnostic carbohydrates is performed by fluorophore assisted carbohydrate electrophoresis. Physiological conditions of particular interest that may be ascertained by the subject invention include carbohydrate metabolism diseases, autoimmune diseases, neoplasia, toxic chemical exposure and microbial infections. Fluorophore assisted carbohydrate electrophoresis diagnosis may be applied to various patient specimens, including blood, urine and skin.

REFERENCES:
P. Jackson and G. R. Williams "Polyacrylamide gel electrophoresis of reducing saccharides labelled with fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid: Application to the enzymological structural analysis of oligosaccharides" Electrophoresis 1991, 12, 94-96.
Peter Jackson "Polyacrylamide Gel Electrophoresis of Reducing Saccharides Labelled with the Fluorophore 2-Aminoacridone:Subpicometer Detection Using an Imaging System Based on a Cooled Charge-Coupled Device" Analytical Biochemistry 196, 238-244 (1991).

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