Fluorometry method and apparatus using a semiconductor laser dio

Details Fluorometry method and apparatus using a semiconductor laser dio Fluorometry method and apparatus using a semiconductor laser dio

1991-05-03

1993-03-23

Hannaher, Constantine

Radiant energy

Luminophor irradiation

2504591, 356318, G01N 2164

Patent

active

051967090

ABSTRACT:
A method and apparatus for detecting the change in phase angle and/or modulation of emitted fluorescence of a fluorophore excited by modulated light from a laser diode. The light is both monochromatic and coherent, and can contain harmonic frequency components. The laser diode can be used in frequency-domain or phase-modulation measurements of fluorescence, using either phase or modulation techniques. The present invention is an improvement of the prior use of a frequency-doubled, cavity-dumped, dye laser in fluorometers. The invention provides an inexpensive light excitation source that is small in size, easily manageable, allows for short measurement times, and has lower power requirements.

REFERENCES:
patent: 4895156 (1990-01-01), Schulze
Reid et al "Measurement of the Transition Strength of . . . Sequence Band in CO.sub.2 . . . " App. Phys. 17, No. 4, Dec. 1978, pp. 349-353.
Vo-Dinh et al "Phase resolved . . . Fluoroimmunosensor" App. Spect. vol. 44, No. 1, Jan. 1990, p. 128.

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