Fluorometric method of quantitative cell mutagenesis

Details Fluorometric method of quantitative cell mutagenesis Fluorometric method of quantitative cell mutagenesis

1980-12-12

1982-08-17

Smith, William F.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 6, 424 7, 427 4, G01N 130

Patent

active

043450270

ABSTRACT:
A method for assaying a cell culture for mutagenesis is described. A cell culture is stained first with a histochemical stain, and then a fluorescent stain. Normal cells in the culture are stained by both the histochemical and fluorescent stains, while abnormal cells are stained only by the fluorescent stain. The two stains are chosen so that the histochemical stain absorbs the wavelengths that the fluorescent stain emits. After the counterstained culture is subjected to exciting light, the fluorescence from the abnormal cells is detected.

REFERENCES:
Chem. Abs. vol. 85, 1976, 85:188687m.
Nature vol. 260, 1976, pp. 448-451.
Nature vol. 251, 1974 pp. 156-158.
Biochemical Genetics 1:61-64 (1967).
Pioc. Nat. Acad.-Sci. USA vol. 72, No. 2, pp. 493-497 2-75.

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