Fluorometric assay of allergic reactions

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 21, 436513, 436809, G01N 33053

Patent

active

048450278

ABSTRACT:
A method for identifying and quantifying allergen specific IgE levels in patient serum by conjugating the serum IgE with allergens on an insoluble support, conjugating the serum IgE with an enzyme labeled anti-IgE antibody, contacting the enzyme with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme, and measuring the level of fluoresence in the solution.

REFERENCES:
patent: Re31006 (1982-08-01), Schuurs et al.
patent: 3720760 (1973-03-01), Bennich
patent: 3941876 (1976-03-01), Marinkovich
patent: 4002532 (1977-01-01), Weltman
patent: 4211762 (1980-07-01), Huggins
patent: 4240751 (1980-12-01), Linnecke
patent: 4331650 (1982-05-01), Brewer
patent: 4347311 (1982-08-01), Schmitz
patent: 4376110 (1983-03-01), David et al.
patent: 4450231 (1984-05-01), Ozkan
patent: 4501970 (1985-02-01), Nelson
"Enzyme-Immunoassay", Maggio, E. T., ed., pp. 26, 173-178, 186, CRC Press, Inc., Boca Raton, 1980.
"Automated Immunoanalysis", Part 2, R. F. Ritchie, ed., pp. 335-342, Marcel Dekker, Inc., New York, 1978.
Poulson et al., Vox Sang., 23:107-118, (1972).
Poulson et al., BIOCHEMICA ET BIOPHYSICA ACTA, 82:463-475, (1964).
Voller, A., et al.; Brief Communication, WHO 51:209-211, (1974).
Voller, A., Chapt. 9, "Heterogenaous Enzyme-Immunoassays and the Applications", pp. 181-196, in Enzyme Immunoassay, (E. Maggio, ed., CRC Press, Boca Raton, Fla., 1980).
Shalev, A., et al.; J. Immuno. Methods, 38:125-139, (1980).
Hellsing, K., et al., Chapt. 3, pp. 67-112, Automated Immunoanalysis (Marcel Dekker, New York), 19.
Ceska, M., et al., Eur. J. Immunol., 2:58-62, (1972).
Bulter, J. E., Chapt. 2, "Antibody-Antigen and Antibody"--Hapten Reactions, pp. 5-52, in Enzyme Immunoassay, (E. Maggio, Ed., CRC Press, Buca Raton, Fla., 1980).
Clark, B. et al., Chapt. 8, "Enzyme Linked Immunosorbent Assay (ELISA): Theoretical and Practical Aspects", pp. 167-179 in Enzyme Immunoassay, (E. Maggio, Ed., CRC Press, Boca Raton, Fla., (1980).
Mattiasson, B., et al., Chapt. 11, "Novel Approaches to Enzyme Immunoassay", pp. 213-248 in Enzyme Immunoassay, (E. Maggio, Ed., CRC Press, Boca Raton, Fla., 1980).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Fluorometric assay of allergic reactions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorometric assay of allergic reactions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorometric assay of allergic reactions will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-852070

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.