Fluorometric assay for measurement of antioxidant activity

Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence

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436 60, 436 71, 436 86, G01N 2164

Patent

active

061141771

ABSTRACT:
A method for measuring total antioxidant activity of antioxidants utilizes BODIPY dyes which fluoresce in the visible region of the electromagnetic spectrum as a fluorescent reporter.

REFERENCES:
patent: 4269938 (1981-05-01), Frank
patent: 4774339 (1988-09-01), Haugland et al.
patent: 5338854 (1994-08-01), Kang et al.
patent: 5372936 (1994-12-01), Fraatz et al.
patent: 5395755 (1995-03-01), Thorpe et al.
patent: 5460943 (1995-10-01), Hayashi et al.
patent: 5554502 (1996-09-01), Mithuhashi et al.
patent: 5605809 (1997-02-01), Komoriya et al.
patent: 5614386 (1997-03-01), Metzker et al.
patent: 5762930 (1998-06-01), Fanger et al.
patent: 5804395 (1998-09-01), Schade et al.
Holler, Tod. P.; Hpkins, Paul B. "A Qualitative Fluorescence-Based Assay for Tyrosyl Radical Scavenging Activity: Ovothiol A is an Efficient Scavenger." Analytical Biochemistry, 326-330, Aug. 1989.
Tsuchiya, M.; Scita, G.; Freisleben, H.; Kagan, V.; and Packer, L. "Antioxidant Radical-Scavenging Activity of Carotenoids and Retinoids Compared to alpha-Tocopherol." Methods in Enzymology, 460-472, 1992.
Karolin, J; Johansson, L.; Strandberg, L.; and Ny, T. "Fluorescence and Absorption Spectroscopic Properties of Dipyrrometheneboron Difluoride (BODIPY) Derivatives in Liquids, Lipid Membrances, and Proteins." J. Am. Chem. Soc., 7801-7806, Aug. 1994.
Park, D. K.; and Song, J. H. "Determination of the Antioxidant Activity of Vitamins C and E According to Lipid Physical State Using High Performance Liquid Chromatography with Chemiluminescence Detection." Korean Biochem. J., 473-478, 1994.
Makrigiorgos, G. M. "Detection of Lipid Peroxidation on Erythrocytes Using the Excimer-Forming Property of a Lipophilic BODIPY Fluorescent Dye." Biochem. and Biophys. Methods, 23-35, Aug. 1997.
Kang, M. Y.; Tsuchiya, M.; Packer, L.; and Manabe, M. "In Vitro study on Antioxidant Potential of Various Drugs Used in the Perioperative Period." Acta Anaesthesiologica Scand. 4-12, 1998.

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