Fluorometric assay composition for measurement of antioxidant ac

Chemistry: analytical and immunological testing – Lipids – triglycerides – cholesterol – or lipoproteins

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436172, G01N 2164

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active

060603240

ABSTRACT:
A method for measuring total antioxidant activity of antioxidants utilizes BODIPY dyes which fluoresce in the visible region of the electromagnetic spectrum as a fluorescent reporter.

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Makrigiorgos, G.M. "Detection of lipid peroxidation on erthrocytes using the excimer-forming property of a lipophilic BODIPY fluorescent dye" J. Biochem. Biophys. Methods, vol. 35, 23-35 (1997).
Karolin, J. et al "Fluorescence and Absorption Spectroscopic Properties of Dipyrrometheneboron Difluoride (BODIPY) Derivatives in Liquids, Lipid Membranes, and Proteins" J. Am. Chem. Soc. vol. 116, 7801-7806 (1994).
Tsuchiya, M. et al "Antioxidant Radical-Scavenging Activity of Carotenoids and Retinoids Compared to alpha-Tocopherol" Methods in Enzymology, vol. 213, 460-472 (1992).
Haugland, R.P. "Molecular Probes: Handbood of Fluorescent Probes and Research Chemicals" 5th ed. pp. 267-272 (1992).

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