Fluorometric assay

Details Fluorometric assay Fluorometric assay

1983-02-24

1985-01-22

Richman, Barry S.

Chemistry: analytical and immunological testing

Optical result

With fluorescence or luminescence

2504611, 436501, 436537, 436800, G01N 2117, G01N 2125

Patent

active

044952937

ABSTRACT:
A method is provided to fluorometrically determine a ligand in an assay solution containing the ligand, reagent system and a fluorescer wherein the intensity of the fluorescer emitted by the assay solution is related to the change in the transmittive properties of the assay solution produced by the interaction of the ligand to be determined and a reagent system capable of producing a change in the transmittive properties of the assay solution in the presence of the ligand. In addition, novel reagent compositions are provided which may be utilized to either spectrophotometrically or fluorometrically determine the concentration of a ligand in an assay solution.

REFERENCES:
patent: 3874853 (1975-04-01), Byrnes
patent: 4095948 (1978-06-01), Hunziker
patent: 4161515 (1979-07-01), Ullman
patent: 4261968 (1981-04-01), Ullman et al.
patent: 4273556 (1981-06-01), Gindler
Willard et al., Instrumental Methods of Analysis, 4th Edition, (1968), pp. 370-395.
Tietz, Fundamentals of Clinical Chemistry, W. B. Saunders, (1970), pp. 87-98.
Tietz, supra, pp. 107-108; pp. 139-142.

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