Fluorometer with low heat-generating light source

Details Fluorometer with low heat-generating light source Fluorometer with low heat-generating light source

2005-02-08

2005-02-08

Porta, David (Department: 2878)

Radiant energy

Luminophor irradiation

C250S483100, C250S462100, C250S459100, C250S461200

Reexamination Certificate

active

06852986

ABSTRACT:
This invention concerns a fluorometer preferably combined with a thermal cycler useful in biochemical protocols such as polymerase chain reaction (PCR) and DNA melting curve analysis. The present flourometer features a low heat-generating light source such as a light emitting diode (LED), having a one-to-one correspondence to each of a plurality of sample containers, such as capped PCR tubes in a standard titer tray. The flourometer of the present invention further comprises an optical path between each LED and its correspondingly positioned container, and another optical path between each flourescing sample within the positioned container and an optical signal sensing means. The instrument can be computer controlled.

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