Fluorometer having an improved optical system

Details Fluorometer having an improved optical system Fluorometer having an improved optical system

1983-08-29

1985-08-20

Smith, Alfred E.

Radiant energy

Luminophor irradiation

With ultraviolet source

G01L 120

Patent

active

045366551

ABSTRACT:
A fluorometer system is disclosed in which a carousel-like system is rotated by a stepper motor to provide a sequence of sample wells to a single optical exciting path. An ultraviolet tube positioned adjacent that path provides an excitation beam which is reflected into common optical path which coincides with the position of the well along the common path is deflected by a beam splitter or the like through an appropriate filtering device to a photomultiplier tube which is located adjacent the common light path on the same side of the path as the ultraviolet tube. The photomultiplier tube is located below the ultraviolet tube to provide a proper direction of convection current.
Further, a solid state photosensor sensitive in red spectrum such as a phototransistor or photodiode is provided adjacent the ultraviolet tube, with a ruby positioned between the ultraviolet tube and the photosensor the take advantage of the fact that solid-state photosensors are especially responsive to light in the red range. The output of the photosensor is fed back to the A to D converter and microprocessor software to take account for the fact that with tube aging or fluctuation in the line current, the output of the ultraviolet tube may vary.

REFERENCES:
patent: 3854050 (1974-12-01), Peterson et al.
patent: 4258264 (1981-03-01), Kotera et al.
patent: 4394580 (1983-07-01), Gielisse

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