Fluoroketone enzyme inhibitors

Details Fluoroketone enzyme inhibitors Fluoroketone enzyme inhibitors

1993-03-22

1994-09-06

Ramsuer, Robert W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Phosphorus esters

536 179, 536 184, 558198, 560130, 560253, 560266, C07F 909, C07F 912, C07H 1504, C07H 15203

Patent

active

053449525

ABSTRACT:
A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two enzymes and a blocked modulator for one of the enzymes. Ligand present in the liquid binds to an antiligand and an enzyme-labeled tracer. The resulting bound fraction is separated and the enzyme in the tracer removes the blocking group from the blocked modulator. The modulator activates or inhibits a second enzyme which catalyzes the conversion of a substrate to a product. The presence or absence of the ligand in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the product. The invention includes a new class of enzyme inhibitors and blocked inhibitors and a kit of materials useful for performing the method of the invention.

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