Fluorogenic substrates for measurement of lysosomal enzyme activ

Details Fluorogenic substrates for measurement of lysosomal enzyme activ Fluorogenic substrates for measurement of lysosomal enzyme activ

1990-03-30

1992-02-18

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 18, 435 19, 435 29, 536 187, 536 55, 536119, 514524, 514525, 560138, 560142, 560145, C12Q 142, C12Q 102, G01N 3100, G01N 3508

Patent

active

050893920

ABSTRACT:
Fluorogenic substrates and compositions containing the same are useful for detecting enzymatic activity within intact cells. The fluorogenic substrates are lysosomotropic derivatives of 2,3-dicyano-hydroquinone (DCH). These derivatives may be employed for detecting lysosomal enzymatic activity within intact cells using fluorescent microscopy and cytometry.

REFERENCES:
patent: 3950322 (1976-04-01), Thomas et al.
patent: 4242447 (1980-12-01), Farid et al.
patent: 4659657 (1987-04-01), Harnisch et al.
patent: 4818692 (1989-04-01), Rauscher et al.
patent: 4959301 (1990-09-01), Weaver et al.
Chemical Abstracts (1988) 109:139297q.
Chemical Abstracts (1989) 110:31853z.
Huxham et al., (1986) A Simple Visual Method for Assessing the Activation and Inhibition of Phenoloxidase Production by Insect Haemocytes in Vitro, J. Immuno Meth 94:271-7.
Musgrove et al., "Flow Cytometric Measurement . . . ," Cytometry, vol. 7, (1986), pp. 347-355.
Bieberich et al., "Intracellular Activity of Lysosomal . . . ," Biol. Chem. Hoppe-Seyler, vol. 70, (1989), pp. 809-817.
Jongkind et al., "Detection of Acid-.beta.-Galactosidase . . . ," Cytometry, vol. 7, (1986), pp. 463-466.
Wittrup et al., "A Single-Cell Assay . . . ," Cytometry, vol. 9, (1988), pp. 394-404.
de Duve et al., "Lysosomotropic Agents," Biochemical Pharmacology,, vol. 23, (1974), pp. 2495-2531.
Firestone et al., "Lysosomotropic Agents . . . ," J. Med. Chem., vol. 22, (1979), pp. 1130-1133.
Brown et al., "Effect on pH on the Emission . . . ," J. Chem. Soc. Farid, Trans. I, (1977), pp. 1281-1285.
Dolbeare "Dynamic Assay of Enzyme Activity . . . ," J. Histochem. Cytochem., vol. 27, No. 12, (1979), pp. 1644-1646.
Gillies et al., "Flow Cytometric Analysis . . . ," Amer. J. Physiol., vol. 253, (1987), pp. C121-C125.
Ohkuma et al., "Fluorescence Probe Measurement . . . ," Proc. Natl. Acad. Sci. U.S.A., vol. 75, No. 7, Jul. 1978, pp. 3327-3331.
Neufeld et al., Annual Review of Biochemistry, Inherited Disorders of Lysosomal Metabolism, vol. 44, (1975), pp. 357-376.

Affiliated with

Also associated with

Rating

Fluorogenic substrates for measurement of lysosomal enzyme activ does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorogenic substrates for measurement of lysosomal enzyme activ, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorogenic substrates for measurement of lysosomal enzyme activ will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1822947