Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1997-02-20
2000-03-14
MacMillan, Keith D.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 24, 435 772, 436164, 436172, 530300, 530350, C12Q 137, G01N 2100, G01N 2176, A61K 3800
Patent
active
060371374
ABSTRACT:
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone each end of which is conjugated to a fluorophore. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high fluorescence signal in the visible wavelengths, these protease indicators are particularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.
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Komoriya Akira
Packard Beverly S.
MacMillan Keith D.
Oncoimmunin, Inc.
Ricigliano Joseph W.
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