Fluorogenic materials and uses thereof

Details Fluorogenic materials and uses thereof Fluorogenic materials and uses thereof

2003-10-15

2009-12-08

Tsang, Cecilia (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

C530S324000, C530S334000, C530S345000, C530S350000, C435S004000, C435S023000, C435S024000, C435S034000, C525S050000, C525S054100, C549S288000

Reexamination Certificate

active

07629437

ABSTRACT:
A method is presented for the preparation and use of fluorogenic peptide substrates that allows for the configuration of general substrate libraries to rapidly identify the primary and extended specificity of enzymes, such as proteases. The substrates contain a fluorogenic-leaving group, such as 7-amino-4-carbamoylmethyl-coumarin (ACC). Substrates incorporating the ACC leaving group show comparable kinetic profiles as those with the traditionally used 7-amino-4-methyl-coumarin (AMC) leaving group. The bifunctional nature of ACC allows for the efficient production of single substrates and substrate libraries using solid-phase synthesis techniques.

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