Fluorogenic enzyme substrates and methods of preparation

Details Fluorogenic enzyme substrates and methods of preparation Fluorogenic enzyme substrates and methods of preparation

2011-01-04

2011-01-04

McIntosh, III, Travis C (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S008000, C536S018100

Reexamination Certificate

active

07863428

ABSTRACT:
This invention relates to hydrolase fluorogenic substrates with improved cell permeability, methods for the preparation thereof, and methods of measuring activities of hydrolases, particularly in cell-based assays. The substrates easily diffuse into the cells, where they are enzymatically processed to yield photostable fluorescent products, and are particularly fitted for visualising enzyme-derived activities in cell-based assays.

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