Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1994-04-14
1996-05-07
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435 47, 435 49, 435174, 435177, 435810, 540221, 540222, 514201, 514202, C12Q 134, C07D50134
Patent
active
055145612
ABSTRACT:
Chromogenic and fluorogenic substrates for .beta.-lactamase, methods for synthesis thereof and methods for detecting .beta.-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent .beta.-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by .beta.-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.
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Hoke Randal A.
Millner, deceased O. Elmo
Mize Patrick D.
Quante J. Michael
Woodard Daniel L.
Becton Dickinson and Company
Fugit Donna R.
Mohamed Abdel A.
Wityshyn Michael G.
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