Fluorogenic and chromogenic .beta.-lactamase substrates

Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...

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540227, 540222, C07D50120, C07D50126, C07D50136, C07D50134

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active

055832172

ABSTRACT:
Chromogenic and fluorogenic substrates for .beta.-lactamase, methods for synthesis thereof and methods for detecting .beta.-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent .beta.-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by .beta.-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.

REFERENCES:
patent: 5189157 (1993-02-01), Wei et al.
patent: 5338843 (1994-08-01), Quante et al.

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