Fluorimetric demonstration and determination of a reduced coenzy

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23230B, G01N 3114

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active

040973387

ABSTRACT:
The present invention relates to a rapid and sensitive method for the determination of a reduced coenzyme, particularly NADH, NADPH and derivatives thereof, in that the fluorescence of such a reduced coenzyme in an aqueous medium is measured in the presence simultaneously of an organic liquid miscible with water and a dipersion of one or more slightly soluble or insoluble substances.

REFERENCES:
patent: 3384555 (1968-05-01), Guilbault et al.
patent: 3637655 (1972-01-01), Clendenning
patent: 3660240 (1972-05-01), Chappelle et al.
patent: 3746625 (1973-07-01), Bergmeyer et al.
patent: 3764478 (1973-10-01), Bergmeyer et al.
Lowry et al., J. Biol. Chem., vol. 224 (1957), pp. 1047-1064.

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