Fluorescent tag for sugar electrophoresis

Details Fluorescent tag for sugar electrophoresis Fluorescent tag for sugar electrophoresis

1990-02-16

1991-07-30

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041828, 204299R, 2041801, 536127, C07H 106, C07H 300, C25B 700

Patent

active

050357867

ABSTRACT:
A method of separating mixtures of saccharides into distinct detectable groups is disclosed. The method comprises modifying 1-amino-4-naphthalene sulfonic acid (ANSA) with a light-sensitive azido-group and binding the modified ANSA to saccharides to form ANSA/saccharide conjugates. The conjugates are subjected to electrophorectic separation to obtain separate groups of conjugates in the gel. The groups of conjugates are transferred from the gel to the surface of a membrane which is exposed to light for a sufficient time and light frequency to activate the azido-group. The light-activated azido-groups attached to the surface of the membrane. The ANSA/saccharides conjugates can then be contacted with labeled probes such as radiolabeled proteins to determine the affinity of the probes to particular saccharides.

REFERENCES:
patent: 4109075 (1978-08-01), Deaton
patent: 4305799 (1981-12-01), Schwarz et al.
patent: 4666581 (1987-05-01), Itoh et al.
Takara Shuzo Company, Ltd.; Central Research Labs, Kyoto, Japan, Carbohydrate Analysis System Catalog--13 pgs.

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