Fluorescent tag for sugar electrophoresis

Details Fluorescent tag for sugar electrophoresis Fluorescent tag for sugar electrophoresis

1990-09-27

1992-02-11

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041828, 204299R, 536127, G01N 2726

Patent

active

050873377

ABSTRACT:
A method of separating mixtures of saccharides into distinct detectable groups is disclosed. In accordance with the method a tri-functional conjugate must first be provided. The tri-functional conjugate is obtained by reacting a mixture of saccharides with moieties (a) capable of providing a charge upon ionization; (b) capable of fluorescing under ultraviolet light; and (c) having a light-activatable azido group thereon. The different functional moieties may all be present on the same moiety and connected directly to the saccharide or may be connected to each other wherein only one of the moieties is connected directly to the saccharide. The tri-functional conjugates are subjected to electrophoretic separation to obtain separate groups of conjugates in the gel. The groups of conjugates are transferred from the gel to the surface of a membrane which is exposed to light for a sufficient time and light frequency to activate the azido-group. The light-activated azido-groups attached to the surface of the membrane. The tri-functional conjugates can then be contacted with labeled probes such as radiolabeled proteins to determine the affinity of the probes to particular saccharides.

REFERENCES:
patent: 4109075 (1978-08-01), Deaton
patent: 4305799 (1981-12-01), Schwarz et al.
patent: 4666581 (1987-05-01), Itoh et al.

Affiliated with

Also associated with

Rating

Fluorescent tag for sugar electrophoresis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescent tag for sugar electrophoresis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent tag for sugar electrophoresis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-780151