Fluorescent substrates for detecting organophosphatase...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C549S220000

Reexamination Certificate

active

07374900

ABSTRACT:
Disclosed are compounds of the formula (I): wherein R3, R4, R5, R9, and R10are selected from the group consisting of H and groups or atoms other than H, and R6and R8are halo or hydrogen; X1, X2, and X3are independently O or S; provided that R9and R10are not simultaneously H, when all of X1, X2, and X3are O; and of the formula (II) wherein R11-R14are selected from the group consisting of H and groups or atoms other than H; X4-X9are independently O or S; n and m are 0 or 1 but m and n cannot be 0 simultaneously; R15-R24can be H or any substituent so long as the compound of formula II upon hydrolysis provides a fluorescent compound. These compounds are useful as substrates with high specificity for organophosphatase particularly human paraoxonase and bacterial organophosphorus hydrolase. Also disclosed is a method for detecting and/or measuring the paraoxonase activity in a fluid comprising contacting the fluid with a fluorescent substrate and measuring the fluorescence of the fluorescent product formed.

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