Fluorescent stokes shift probes for polynucleotide hybridization

Details Fluorescent stokes shift probes for polynucleotide hybridization Fluorescent stokes shift probes for polynucleotide hybridization

1990-04-13

1991-02-26

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 436800, 536 27, 935 78, C12Q 168

Patent

active

049961438

ABSTRACT:
Fluorescent stokes shift probes for polynucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non-radiative energy transfer are separated by 2 to 7 nucleotide base units. The fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.

REFERENCES:
patent: 4542104 (1985-09-01), Stryer et al.
patent: 4584277 (1986-04-01), Ullman
patent: 4666862 (1987-05-01), Chan

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