Fluorescent spectral differential measurement of a substrate coa

Details Fluorescent spectral differential measurement of a substrate coa Fluorescent spectral differential measurement of a substrate coa

1994-02-08

1995-11-07

Fields, Carolyn E.

Radiant energy

Luminophor irradiation

Methods

250302, 2504581, 2504611, G01N 2164

Patent

active

054649862

ABSTRACT:
A fluorescent spectrophotometer measures the fluorescent characteristics of fluorescent probes within functional compositions of a specimen. The fluorescent spectrophotometer emits light appropriate for exciting the fluorescer or fluorescers contained within the specimen. The excited fluorescer emits within an emission wavelength spectrum. Fluorescers having a narrower wavelength region over which an abrupt change in emission intensity occurs are chosen for use as a fluorescent probe. The fluorescent spectrophotometer detects the change in intensity through this narrower area and performs a derivative calculation on the intensity curve. The fluorescent spectrophotometer is able to distinguish the fluorescer from other fluorescers or interfering fluorescent backgrounds.

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